BES; Free Acid; ULTROL Grade

Code: 391334-100GM D2-231

General description

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: ﹤0.05 at 260 nm, 100 mM). Chen ...


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Your Price
€223.00 100GM
€274.29 inc. VAT

General description

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: ﹤0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C.

Absorbance (100 mM, 260 nm):≤0.05.

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.

Legal Information

ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

De Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.Chen, C.A., and Okayama, H. 1988. Biotechniques6, 632.

Packaging

100 g in Glass bottle

25 g in Plastic ampoule

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.

Warning

Toxicity: Standard Handling (A)

assay≥99% dry basis (titration)
cation tracesheavy metals: ≤1 ppm
colorwhite
formsolid
InChI keyAJTVSSFTXWNIRG-UHFFFAOYSA-N
InChI1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
manufacturer/tradenameCalbiochem®
pKa (20 °C)7.12
Quality Level100
shipped inambient
solubilitywater: 1 mg/mL
storage conditionOK to freeze
storage temp.15-25°C
Cas Number10191-18-1
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