General description
BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: ﹤0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C.
Absorbance (100 mM, 260 nm):≤0.05.
BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.
Legal Information
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
De Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.Chen, C.A., and Okayama, H. 1988. Biotechniques6, 632.
Packaging
100 g in Glass bottle
25 g in Plastic ampoule
Reconstitution
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.
Warning
Toxicity: Standard Handling (A)
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